Abstract: Marine biological resource is diverse and one of the world's largest carbon storage treasure house. However, long-term reliance on traditional farming and fishing methods with high energy consumption and pollution has resulted in a large amount of energy consumption, increased carbon emissions from fisheries, and further deterioration of the fishery ecological environment, posing new challenges for the low-carbon development of marine fisheries. In order to solve the problems mentioned above, based on the PVAR model, this paper empirically analyzed the relationship among environmental regulation, technological innovation and marine fishery total factor productivity, and drew the following conclusions: environmental regulation and technological innovation could promote marine fishery total factor productivity, but the marine fishery total factor productivity was affected by environmental regulation and innovation of science and technology with obvious regional differences. In 11 provinces (municipalities), the Yellow Sea and Bohai Sea region, and the Pearl River Delta region, the contribution of environmental regulation on marine fishery total factor productivity was greater than the contribution of science and technology innovation on the marine fishery total factor productivity, while the Yangtze River delta and vice versa. However, the impact of marine fishery total factor productivity on environmental regulation and technological innovation was a temporary promotion with a certain incubation period. When it came to environmental regulation and scientific and technological innovation, the interaction between the two was not equal.